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By Svec Frantisek

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As of today, UV detection is mostly used to monitor the separations. Unfortunately, on-column detection is required in CEC to avoid excessive peak broadening and loss of the high intrinsic efficiency of this method. Therefore, the capillary diameter is the only light pass length available for detection. This situation dramatically impairs the sensitivity of the UV detection. Several attempts to address this weakness such as the high sensitivity Z-cell developed by Agilent Technologies did not meet with a broad acceptance.

1 Characteristics of the SMC . . . . . . . . . . . 69 Application of SMC for Liquid Chromatography of Biomolecules . 72 Plasmid DNA Purification . . . . . . . . . . . 75 5 Other Applications of SMC . . . . . . . . . . . 3 Use of SMC as Biosensors and for Fast Bioconversion . . . . 80 SMC for Solid Phase Extraction or Fast Sample Clean Up . . . 81 Use of SMC for Solid Phase Synthesis . . . . . . . . 81 6 Conclusions and Perspectives .

Silica Based Monoliths . . . . . . . Soft Organic Gel-Based Monoliths . . . . Rigid Organic Gel-Based Monoliths . . . . 2 Theoretical Background . . . . . . . . . . . . 3 Early Results About the Influence of Column Length on Resolution 58 The Concept of SMC . . . . . . . . . . . . . 60 Resolution and Efficiency in SMC-Chromatography . . . . 62 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51 52 53 54 55 55 Advances in Biochemical Engineering/ Biotechnology, Vol.

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