By P.M. DEY (Eds.)
The sequence, Methods in Plant Biochemistry, presents an authoritative reference on present strategies within the quite a few fields of plant biochemical study. each one quantity within the sequence will, lower than the professional counsel of a visitor editor, care for a specific workforce of plant compounds. each one will describe the ancient heritage and present, most precious tools of study. The volumes contain particular discussions of the protocols and suitability of every method. Case remedies, diagrams, chemical buildings, reference info, and homes should be featured besides an entire checklist of references to the expert literature.
Conceived as a pragmatic better half to The Biochemistry of vegetation, edited by means of P.K. Stumpf and E.E. Conn, no plant biochemical laboratory can come up with the money for to be with out this accomplished and up to date reference resource
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Additional info for Carbohydrates
The high water-mark of nucleotide sugar research was reached in the late 1970s, and the number of new publications concerned with these compounds has been constantly decreasing. Since they are connected with such a large variety of metabolic processes, nucleotide sugars have now become common laboratory reagents and are used almost daily in many investigations. Nonetheless, in the realm of plant biochemistry some intriguing unsolved problems involving nucleotide sugars remain. Perhaps the most interesting and enigmatic of these is the biosynthesis of the L-arabinofuranosyl moiety.
Sodium acetate. 3. Trifluoroacetic anhydride. Procedure: Samples (1 mg) are added to Teflon-capped vials. If the samples are in aqueous solution, they should first be taken to dryness in a stream of dry nitrogen. 1 ml). The capped vials are heated at 60°C for 1 h, after which the water is evaporated in a stream of nitrogen. 1 ml) is added, and samples are again taken to dryness. Traces of water are removed by addition of benzene, evaporated as the azeotrope. 015 ml) are added. The capped vials are kept at room temperature for 2 h or at 4°C for 12 h.
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