Download Extracellular Nucleic Acids by Wen-Chi Chiang, Tim Tolker-Nielsen (auth.), Yo Kikuchi, PDF

By Wen-Chi Chiang, Tim Tolker-Nielsen (auth.), Yo Kikuchi, Elena Y. Rykova (eds.)

Extracellular nucleic acids have lately emerged as very important gamers within the fields of biology and the scientific sciences. within the final numerous years, extracellular nucleic acids were proven to be eager about not just microbial evolution as genetic components but in addition to have structural roles in bacterial groups, reminiscent of biofilms. Circulating DNA and RNA were present in human blood and anticipated to be valuable as non-invasive markers for the analysis of a number of ailments. additionally, extracellular nucleic acids have attracted cognizance as energetic modulators of the immune method of upper organisms, together with people. This e-book covers the vast majority of the newly constructing fields on the topic of extracellular nucleic acids, together with these of uncomplicated biology, ecology and the clinical sciences, and offers readers with the most recent wisdom on them.

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35 Abstract Extracellular DNA in the natural environment is a source of nutrients and gene pools for bacteria. In sediments and biofilms, gene transfer must occur among bacteria via extracellular DNA because the concentrations of both exogenous DNA and cells are high. Some bacteria actively release naked DNA or the membrane vesicle containing the DNA into the environment. Some bacteria also produce extracellular DNA by suicide and fratricide.

49 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51 Abstract Molecular biology technology generally requires naked DNA purified in a test tube. However, extracellular DNA in the natural environment can contribute to breeding and growth as nutrition or genetic information. Transfer and maintenance of DNA is called horizontal gene transfer (HGT), a mechanism that has certainly played a role during evolution.

3 Why Is Natural Transformation Important for Molecular Biology Research and Application? . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 Production of Stable Extracellular DNA Released from Donor Cells . . . . . . . . . . 1 Extracellular DNAs Released from Cells Lysed in the Laboratory . . . . . . . 2 Extracellular DNA: An Active Substrate in the Natural Transformation of a Gram-Positive Bacterium . . . . . . . . . . . . . . . . .

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